OBJECTIVE： To optimize the extraction technology of Tibetan medicine Pedicularis kansuensis and compare content of verbascoside and isoverbascoside differences in P. kansuensis from various habitats. METHODS： Using verbascoside and isoverbascoside and dry paste yield as comprehensive evaluation indexes， single factor test and orthogonal test were used to investigate the extraction solvent， solvent dosage， extraction time and times to optimize extraction technology， and the verification test was conducted. Contents of the 2 constituents verbascoside and isoverbascoside in P. kansuensis from Gansu， Qinghai and Sichuan were compared. RESULTS： The optimal extraction technology was as follows as 8-fold 50％ ethanol， extraction for 3 times， 90 min each time. The verification results showed that the average contents of verbascoside and isoverbascoside were 3.49％ （RSD＝1.28％， n＝3）， 1.26％ （RSD＝1.32％， n＝3）， and average dry paste yields were 37.99％ （RSD＝1.97％， n＝3）. The contents of verbascoside and isoverbascoside in P. kansuensis from Qinghai were relatively higher. CONCLUSIONS： Optimized extraction technology is reasonable， stable， feasible； the contents of index constituents in P. kansuensis from different habitats have certain differences. The study can provide scientific evidence for the development and utilization of extraction， and the in-depth study of quality evaluation for medicinal material.