OBJECTIVE： To investigate the effects of gemcitabine combined with radiotherapy and chemotherapy on angiogenesis and cell invasion ability of cervical cancer. METHODS： Totally 37 patients undergoing radical operation selected from Chongqing Kaizhou District People’s Hospital during Jan. 2015-Jun. 2016 were divided into observation group （n＝19） and control group （n＝18）. Control group was given 3-D conformal radiation and chemotherapy which included Cisplatin injection 70 mg/m2，ivgtt+Fluorouracil injection 600 mg/m2，ivgtt on 1st and 21st day after radiotherapy.  Observation group was additionally given Gemcitabine hydrochloride for injection 800 mg/m2，ivgtt， 1st and 8th day after radiotherapy. A treatment course lasted for 28 d. The radical hysterectomy and  pelvic lymphadenectomy were conducted after 2 courses of treatment. Clinical efficacy， microvessel density （MVD） of tumor， vascular endothelial growth factor （VEGF）， Wnt1， Wnt3a， Wnt8 and β-catenin level were observed in 2 groups. The relationship of MVD with VEGF was analyzed， and the occurrence of ADR was recorded. RESULTS： The total response rate of observation group （84.2％） was significantly higher than control group， with statistical significance （P＜0.05）. The levels of MVD， VEGF， Wnt1， Wnt3a， Wnt8 and β-catenin in 2 groups were significantly higher than healthy tissue； above indexes of focus tissue in observation group were significantly lower than control group， with statistical significance （P＜0.05）. MVD was positively correlated with VEGF. There was no statistical significance in the incidence of ADR between 2 groups （31.6％ vs. 27.8％， P＜0.05）. CONCLUSIONS： Gemcitabine combined with radiotherapy and chemotherapy can regulate the angiogenesis of cervical cancer cell and the activity of Wnt/β-catenin signal pathway， and reduce the invasion ability of cervical cancer cell， which may be the important mechanism of anti-tumor effect with good safety.