OBJECTIVE： To compare the total flavonoids content and antioxidant activity in the barks， leaves， male flowers and seeds of Eucommiae ulmoides. METHODS： UV spectrophotometry was used to determine the total flavonoids content in different parts； tests was conducted to clear 2， 2′ -nitrilobis （3-ethylbenzothiazoline-6-sulfonic acid） （ABTS+）， 1， 1-diphenyl-2-trinitrophenylhydrazine （DPPH） radicals and the reducing ability of Cu2+， using half clear/reduction concentration value （IC50） as evaluation indexes， and vitamin C was regarded as positive control. RESULTS： The total flavonoids content of the E. ulmoides from hight to low was as follows as leaves＞male flowers＞barks＞seeds， except there was no significant difference in barks and seeds （P＞0.05）， the other parts had significant differences （P＜0.05）； the ability of different parts eliminating DPPH and ABTS+ free radical was as follows as leaves＞male flowers＞seeds＞barks， except there was no significant difference in the indicators of leaves and male flowers （P＞0.05）， the other parts had significant differences （P＜0.05）； the ability of reducing Cu2+ free radical was as follows as leaves＞male flowers＞barks＞seeds， there was significant difference in leaves and males flowers with barks and seeds （P＜0.05）. CONCLUSIONS： The content of total flavonoids in leaves and male flowers is high， and the antioxidant activity is strong， which has a great prospect of exploitation and utilization to make up for deficiencies in barks of E. ulmoides.