OBJECTIVE： To establish the method for simultaneous determination of 6 components in Lonicera japonica， and to study the content changes of them before and after before and after carbonized. METHODS： UPLC method was adopted. The determination was performed on Agilent Eclipse Plus C18 RRHD column with mobile phase consisting of 0.1％ phosphoric acid solution-acetonitrile （gradient elution） at the flow rate of 0.2 mL /min. The detection wavelength was set at 350 nm， and column temperature was 25 ℃. The sample size was 1 μL. RESULTS： The linear ranges of chlorogenic acid， rutin， galuteolin， isochlorogenic acid A， isochlorogenic acid B and isochlorogenic acid C were 21.2-424 μg（r＝0.999 3）， 1.17-23 μg（r＝0.999 5）， 2.18-43 μg（r＝0.999 8）， 5.10-102 μg（r＝0.999 3）， 2.60-52 μg（r＝0.999 1）， 4.95-99 μg（r＝0.999 8）， respectively. RSDs of precision， stability and repeatability tests were all lower than 2.0％. Recoveries were 97.11％-99.76％（RSD＝1.20％，n＝6）， 95.20％-99.90％（RSD＝2.20％，n＝6）， 95.71％-100.30％（RSD＝2.20％，n＝6）， 95.00％-96.98％（RSD＝0.88％，n＝6）， 96.47％-103.00％（RSD＝2.40％，n＝6）， 95.78％-103.80％（RSD＝3.20％，n＝6）. Compared with before processing， the contents of rutin， isochlorogenic acid B and isochlorogenic acid C in L. japonica were increased along with processing， the contents of chlorogenic acid and isochlorogenic acid A were decreased significantly， while the content of galuteolin had no significant change. CONCLUSIONS： The method is simple， precise， stable and repeatable， and can be used for simultaneous determination of 6 components in L. japonica. Those chemical components have certain changes before and after carbonized.