OBJECTIVE: To establish a method for determining the contents of chlorogenic acid and luteoloside in Lonicerae japonicae， and to explore the shelf life of L. japonicae under ordinary temperature and sealed environment. METHODS： HPLC method was adopted. The determination was performed on Agilent Zorbax SB-C18 （chlorogenic acid）column and Agilent Zorbax SB-Phenyl（galuteolin） with mobile phase consisted of acetonitrile-0.4％ phosphoric acid （13 ∶ 87，V/V）， acetonitrile-0.5％ glacial acetic acid （gradient elution， galuteolin） at the flow rate of 1.0 mL/min. The detection wavelength was set at 327 nm （chlorogenic acid） and 350 nm （galuteolin）. The column temperature was 30 ℃， and sample size was 10 μL. RESULTS： The linear range of chlorogenic acid and galuteolin were 10-100 μg/mL （r＝0.998 6）， 5-50 μg/mL （r＝0.999 3）， respectively. RSDs of precision， stability and reproducibility tests were all lower than 4.0％. Recoveries were 95.78％-99.70％（RSD＝1.46％，n＝6）、96.30％-104.31％（RSD＝2.93％，n＝6）. The contents of chlorogenic acid and galuteolin in roller method，baking method and natural drying method of processed L. japonicae were all decreased by 30％-40％ after stored for 12 months. CONCLUSIONS： The method is simple， precise， stable and repeatable， and can be used for simultanoue determination of chlorogenic acid and luteoloside in L. japonicae. The contents of active components in L. japonicae decrease significantly after stored for 12 months. It is necessary to establish shelf life standard of L. japonicae， so as to guarantee the effectiveness of drug use in the clinic.