OBJECTIVE： To establish the HPLC fingerprint for Blumea balsamifera and its fake B. riparia. METHODS： HPLC was performed on the column of Uitimate-C18 with mobile phase of acetonitrile-0.05％ phosphoric acid （gradient elution） at a flow rate of 0.6 mL/min， detection wavelength was 270 nm， column temperature was 25 ℃， and injection volume was 7 μL. Using quercetin as a reference， Similarity Evaluation Software for Chromatographic Fingerprint of Traditional Chinese Medicine （2004 A edition）was used for the common peaks identification and similarity analysis of 16 batches of B. balsamifera and 5 batches of B. riparia. RESULTS： There were 61 common peaks in the 16 batches of B. balsamifera， similarity degree was 0.931-0.995， which was higher than the similarity degree of 5 batches of B. riparia. CONCLUSIONS： The established fingerprint can provide reference for the identification and quality evaluation of B. balsamifera.