OBJECTIVE： To establish the method for the simultaneous determination of contents of 4 heavy metal elements in cultured Cordyceps militaris. METHODS： The samples were digested by nitric acid heating method and then determined by ICP-MS including radio-frequency power of 1.15 kW， sampling depth of 65 nm， auxiliary gas flow rate of 1.0 L/min， cooling gas flow of 13.0 L/min， temperature of 25 ℃， automatic plasma. RESULTS： The linear ranges of arsenic （As） ，cadmium （Cd）， mercury （Hg） and lead （Pb） were 0-20 μg/mL（r＝0.997 0）， 0-10 μg/mL（r＝0.999 5）， 0-5 μg/mL（r＝0.995 5）， 0-20 μg/mL（r＝0.996 0）， respectively. Detectio limit were 0.128，0.003，0.002，0.004 mg. RSDs of precision， stability and reproducibility tests were all lower than 6.5％. Recoveries of them were 90.4％-100.6％（RSD＝3.45％，n＝9）， 94.3％-101.3％（RSD＝2.93％，n＝9）， 90.0％-102.3％（RSD＝4.03％，n＝9），92.3％-103.0％（RSD＝3.53％，n＝9）， respectively. CONCLUSIONS： The method is simple， precise， stable， reproducible， and can be used for simultaneous determination of contents of 4 heavy metal elements in cultured C. militaris. The contents of As， Cd， Hg and Pb in 10 batches of sample are all in line with related national standards.