OBJECTIVE: To prepare the Albendazole nanoliposomes freeze-dried power and study its properties. METHODS: Freeze-drying method was conducted to prepare Albendazole nanoliposomes freeze-dried power, using the particle size, encapsulation efficiency, appearance, redispersibility as indexes, single factor test was combined with orthogonal test to screen freeze-drying preparation technology. The morphological changes, particle size, Zeta potential, moisture content, 12 months stability at 4 ℃ before and after freeze-drying were detected. RESULTS: Plus a total content of freeze-dried protective agent was 10%, the ratio of glucose-trehalose-mannitol was 1.0 ∶ 1.0 ∶ 3.0, using quick-freeze, pre-freezing 18 h in -35 ℃ refrigerator, dry-freezing 48 h to obtain freeze-dried powder. Compared with before freeze-drying, the freeze-dried liposomal morphology had no obvious changes, showing clear phospholipid bilayer membrane structure; the particle sizes before and after freeze-drying were (208.63±1.04) nm and (223.04±2.02) nm, Zeta potentials were (-15.6±0.04) mV and (-19.4±0.06) mV, encapsulation efficiencies were (94.62±0.49)% and (91.10±0.46)% (n=3), respectively. Compared with liposomes, liposomes freeze-dried power had good stability in 12 months at 4 ℃. CONCLUSIONS: Albendazole nanoliposomes freeze-dried power is prepared successfully, its stability is superior to albendazole nanoliposomes, and the freeze-drying technology is feasible.
关键词
阿苯达唑纳米脂质体冻干粉粒径包封率稳定性正交试验
Keywords
AlbendazoleNanoliposomesFreeze-dried powderParticle sizeEncapsulation efficiencyStabilityOrthogonal test