OBJECTIVE： To provide reference for clarifying the pharmacodynamic material basis of anti-inflammation effect of Uygur medicine Hyssopus cuspidatus. METHODS： HPLC was conducted to establish the fingerprint of 8 extracts with different polarities. Using macrophage RAW264.7 as object，the inhibitory effect of extracts with different polarities on inflammatory factor nitric oxide （NO），TNF-α and IL-6 in supernatant of cell culture medium was detected，the differences of anti-inflammatory activity in vitro were compared. Grey correlation analysis method was used to analyze the spectrum-effect relationship of peak area of common peaks and anti-inflammation activity. RESULTS： The fingerprint of 8 extracts with different polarities showed obvious differences. Anti-inflammation in vitro results suggested that 30％ ethanol extract had the strongest inhibitory effect on inflammatory cytokines in vitro. There were 14 common peaks in the established HPLC fingerprints，the 5 common peaks that was closely related to the inhibitory effect of inflammatory factors in vitro were peak 8，6，5，10，13，respectively；peak 8 was rosmarinic acid. CONCLUSIONS： The established fingerprint and its spectrum-effect relationship with anti-inflammation activity in vitro can provide certain reference for its pharmacodynanic material basis study.