OBJECTIVE： To provide reference for rational use of carbapenem antibiotics in the clinic. METHODS： Enterobacteriaceae bacteria were collected from our hospital during Jan. 2014-Dec. 2015； semiautomatic microbiological assay instrument was used for strain culture， identification and drug sensitive tests. Modified Hodge test and K-B test were adopted to confirm Klebsiella pneumoniae  carbapenemases （KPC）-producing and ESBLs-producing drug resistant strains. RESULTS： During 2014-2015， 1 035 strains of Enterobacteriaceae bacteria were detected in our hospital， among which there were 732 strains of Escherichia coli， 157 strains of K. pneumonia， 136 strains of Enterobacter cloacae and 10 strains of Serratia marcescens. Citrobacter freundii was not found. E. coli and K. pneumonia were highly sensitive to amikacin and carbapenems， but slightly sensitive to most cephalosporin. A total of 64 strains of carbapenems-resistant Enterobacteriaceae bacteria （6.18％） were detected， among which there were 31 strains of carbapenems-resistant E. coli （4.23％）， 30 strains of carbapenems-resistant K. pneumonia （19.11％）， 1 strain of carbapenems-resistant E. cloacae （0.74％） and 2 strains of carbapenems-resistant S. marcescens （20.00％）. The samples were mainly from sputum and urine specimens， which were mainly from neonatal department and ICU. Of 64 drug resistant strains， there were 59 KPC-producing strains （92.19％） and 3 ESBLs-producing strains （4.69％）. CONCLUSIONS： E. coli occupies high proportion among Enterobacteriaceae bacteria， and the number of carbapenems-resistant E. coli and carbapenems-resistant K. pneumoniae is in high level. Drug resistance of Enterobacteriaceae bacteria to carbopenems may be associated with KPC and ESBLs producing. Carbapenem antibiotics should be selected rationally in accordance with medication indications and the results of drug sensitivity test.