OBJECTIVE： To determine residual citrinin in Zhibituo tablet by solid phase extraction-UPLC-MS/MS. METHODS： The sample was processed preliminary by solid extraction. The determination was performed on  Zorbax Eclipse Plus C18 column with mobile phase consisting of water （0.1％ formic acid）-acetonitrile （70 ∶ 30，V/V， gradient elution） at the follow rate of 0.30 mL/min. The column temperature was 40 ℃， and analysis time was 7 min， the sample size was 2 μL. Ionization mode was positive ion mode. The ion source temperature was 150 ℃， drying gas velocity was 15 L/min， sheath gas temperature was 350 ℃，sheath gas flow rate was 12 L/min， capillary voltage was 3 500 V， atomization device  pressure was 40 psi， atomization voltage was 0 V. The acquisition mode was MRM mode. RESULTS： The linear range of citrinin was 0.1-20 ng/mL（r＝0.999 4）； the limits of quantitation and detection were 0.05 and 0.01 ng/mL. RSDs of precision， stability and repeatability tests were all lower than 2.0％； recoveries were 98.868％-103.160％ （RSD＝1.5％，n＝6）. CONCLUSIONS： The method is rapid， sensitive， accurate and suitable for the determination of residual citrinin in Zhibituo tablet.