OBJECTIVE： To determine the content of 17 amino acid in sarcocarp of northeast Empetrum nigrum simultaneously. METHODS： HPLC-ELSD method was adopted. The determination was performed on Waters Symmetry C18 column with mobile phase consisted of 5.0 mmol/L heptafluorobutyric acid solution （containing 0.7％ trifluoroacetic acid）-acetonitrile （gradient elution） at the flow rate of 2.0 L/min， the column temperature was 37 ℃， the drift tube temperature was set at 60 ℃. RESULTS： The line- ar ranges of glycine， serine， aminosuccinic acid， aminopropionic acid， threonine， glutamic acid， cystinol， diaminocaproic acid， histidine， arginine， proline， valine， methionine， oxyphenylaminopropionic acid， isoleucine， aminocaproic acid， phenylalanine were 0.005 9-0.187 7， 0.008 2-0.262 8， 0.010 4-0.332 8， 0.007 0-0.222 7， 0.009 3-0.297 8， 0.011 5-0.367 8， 0.009 4-0.300 4， 0.011 4- 0.365 5，0.012 1-0.388 0，0.013 6-0.435 5，0.009 0-0.287 8，0.009 2-0.293 0，0.011 7-0.373 0，0.014 2-0.453 0，0.010 3-0.328 0， 0.010 3-0.328 0 and 0.012 9-0.413 0 mg/mL （all r＞0.999 0）. RSDs of precision， stability and repeatability tests were not all less than 2.0％. Recoveries were 98.0％-101.2％（RSD＜2.0％，n＝6）. CONCLUSIONS： This method is simple， precision， stable and repeatable， and can be used for simultaneous determination of 17 amino acid in sarcocarp of northeast E. nigrum.