OBJECTIVE： To prepare Ibuprofen lysine sustained-release liquid suppository， and conduct the evaluation in vivo and in vitro. METHODS： Ibuprofen lysine sustained-release liquid suppository was prepared by using Poloxamer solution as main matrix， carbomer as bioadhesive agent， laurocapram as penetrant. The ratios of poloxamer 407 （P407）-poloxamer 188 （P188） and carbomer content were screened by using gel temperature， gel intensity， biological adhesion and release rate in vitro as indexes， and the concentration-time curves and pharmacokinetic parameters of prepared liquid suppository and common solid suppository after rectal administration in Beagle dogs in vivo were compared. RESULTS： When the P407-P188 ratio was 1 ∶ 1.2， the gel temperature of sustained-release liquid suppository was 30.4-38.1 ℃， which was the nearest to the rectal temperature； when the content of carbomer was 0.8％， the in vitro release of sustained-release liquid suppository was in zero-order model， with favorable correlation （R2＝0.996）. The tmax of common solid suppository was 3.206 h， then plasma concentration decreased significantly， release time did not exceed 12 h， AUC0-∞ was 501.826 mg·h/L； tmax of sustained-release liquid suppository was 8.814 h， then plasma concentration decreased， release time exceeded 24 h， AUC0-∞ was 715.489 mg·h/L. CONCLUSIONS： Ibuprofen lysine sustained-release liquid suppository is successfully prepared， which shows better sustained-release effect and excellent correlation in vivo and in vitro than common solid suppository.