OBJECTIVE: To study the chemical constituents in antioxidant activity part of Semiaquilegia adoxoides. METHODS: The antioxidant activity part of S. adoxoides were isolated and purified by chromatography on silicagel and Sephadex LH-20 column, and compound structures were identified physicochemical properties and spectral data analysis. RESULTS: Nine compounds were isolated from the antioxidant activity part of S. adoxoides, namely magnoflorine (1), griffonilide (2), salidroside (3), ferulic acid (4), genistein (5), 2,4-dihydroxybenzoic acid (6), chlorogenic acid (7), caffeic acid (8) and p-coumaric acid (9). CONCLUSIONS: The study confirms the active material basis in antioxidant activity part of S. adoxoides for the first time.