OBJECTIVE： To explore the effect of clinical pharmacist intervention on the rational use of Alanyl-glutamine injection. METHODS： Referring to package inserts of Alanyl-glutamine injection， Clinical Pharmacy Consensus of Parenteral Nutrition， ASPEN Nutrition Therapy Guidelines for Critically Ill Patients， related domestic and foreign literatures， evaluation criteria for Alanyl-glutamine injection rational use was formulated. After collecting Alanyl-glutamine injection cases （497 cases） in the second quarter of 2015 and those cases （385 cases） in the second quarter of 2016， rational use of Alanyl-glutamine injection were analyzed comparatively before and after intervention. RESULTS： The utilization rate and irrational rate of Alanyl-glutamine injection were 4.6％ and 52.9％ before intervention as well as 2.9％ and 10.9％ after intervention， with statistical significance （P＜0.05）. There was statistical significance in hyper-indication， excessive concentration of drug liquid， excessive supply of amino acid， irrational compatibility and solvent selection， long treatment course before and after intervention （P＜0.05）.  CONCLUSIONS： Clinical pharmacists reduce irrational rate of drug use and guarantee safe and effective drug use through formulating evaluation criteria for Alanyl-glutamine injection rational use and providing pharmaceutical intervention on rational use of Alanyl-glutamine injection.