OBJECTIVE： To establish a method for the content determination of loxoprofen silver in Loxoprofen silver cream， and provide reference for the quality control of the preparation. METHODS： HPLC was performed on the column of Diamonsil C18 with mobile phase of methanol-water-glacial acetic acid-triethylamine （55 ∶ 45 ∶ 0.1 ∶ 0.1，V/V/V/V） at a flow rate of 1.0 mL/min， detection wavelength was 223 nm， column temperature was 30 ℃， and the injection volume was 20 μL. RESULTS： The linear range of loxoprofen silver was 6.53-130.7 μg/mL（r＝0.999 9）；the limit of quantitation was 0.253 μg/mL，limit of detection was  0.076 μg/mL； RSDs of precision， stability and reproducibility tests were lower than 2％； recovery was 96.79％-103.68％ （RSD＝2.23％，n＝9）. CONCLUSIONS： The method is simple， accurate and reproducible， and can be used for the content determination of Loxoprofen silver cream.