OBJECTIVE： To virtually screen potential α-glycosidase inhibitor ingredients from C. mori and F. mori， and to provide reference for finding out new type α-glycosidase inhibitor ingredient. METHODS： Surflex-Dock module of Sybyl-x 2.0 molecular simulation software was used to perform the docking of small molecule compound， which was from the ingredients of C. mori and F. mori as ligand stated in literatures， with α-glycosidase. Total score of affinity scoring function was equal to 7 as the threshold value， to judge potential α-glycosidase inhibitor ingredient in C. mori and F. mori. RESULTS： After 70 small molecule compounds docked with α-glycosidase， 10 compounds showed binding activity （Total score≥7.00）. Among them， moracin M-3′ -O-β-D-glucopyranoside， 5，7，2′ -trihydroxyflavanone-4′ -O-β-D-glucoside， mulberroside A， resveratrol-4，3′ -di-O-β-D-glucopyranoside and 1，4-dideoxy-1，4-imino-（2-O-β-D-glucopyranosyl）-D-arabinitol had higher binding activity with α-glycosidase （Total score＞8.00）. CONCLUSIONS： Multi-constituents of C. mori and F. Mori show potential α-glycosidase inhibitory activity. The method is a kind of highly targeted， rapid and efficient approach to discover α-glycosidase inhibitor from traditional Chinese medicine.