OBJECTIVE： To establish the quality standard for Tongkuaixiao oral liquid. METHODS： TLC was used for the qualitative identification of Prunella vulgaris and Polygonum bistorta in the preparation； HPLC was used for the content determination of morphine： the column was Kromsail C18 with mobile phase of acetonitrile-0.1％ phosphoric acid （15 ∶ 85，V/V） （each 100 mL added sodium heptanesulfonate 0.1 g） at a flow rate of 1.0 mL/min， detection wavelength was 220 nm， column temperature was 25 ℃， and injection volume was 10 μL. RESULTS： The TLC spots of P. vulgaris and P. bistorta were clear and well-separated， negative control without interference. The linear range of morphine was 15.13-242.08 μg/mL（r＝0.999 9）； RSDs of precision， stability and reproducibility tests were lower than 2.0％； recovery was 97.80％-102.10％ （RSD＝1.57％，n＝6）. CONCLUSIONS： The establish standard can be used for the quality control of Tongkuaixiao oral liquid.