OBJECTIVE: To develop and improve the quality standard for Kanggu zengsheng tablet. METHODS: TLC was used for the qualitative identification of Drynaria fortunei, Epimedii Folium and Spatholobus suberectus; HPLC was used for the contents determination of icariin and acteoside: the column was Diamonsil C18 with mobile phase of acetonitrile-0.1% formic acid solution (gradient elution) at a flow rate of 1.0 mL/min; detection wavelength was 270 nm for icariin and 334 nm for acteoside, Cdumn temperature was 25 ℃,and the injection volume was 10 μL. RESULTS: The TLC spots of D. fortunei, Epimedii Folium and S. suberectus were clear and well separated, negative control without interference. The linear range was 0.018 8-1.88 μg for acteoside (r=0.999 9) and 0.107-2.14 μg for icariin (r=0.999 9); RSDs of precision, stability and reproducibility tests were lower than 2.0%;recoveries were 100.2%-105.0%(RSD=1.6%,n=9) and 96.2%-99.5%(RSD=1.4%,n=9). CONCLUSIONS: The improved standard can more effectively control the quality of Kanggu zengsheng tablet.