OBJECTIVE： To establish a method for the simultaneous determination of 5 components in Zanthoxylum nitidum. METHODS： HPLC was performed on the column of Luna C18 with mobile phase of acetonitrile-water （containing 0.2％ phosphoric acid， 0.25％ triethylamine） （gradient elution） at a flow rate of 1.0 mL/min， detection wavelength was 273， 284 nm， column temperature was 30 ℃， and the injection volume was 20 μL. RESULTS： The linear range was 0.8-200 μg/mL for allocryptopine （r＝0.999 9）， 0.2-50 μg/mL for sargentine hydrochloride （r＝0.999 9）， 0.44-110 μg/mL for nitidine chloride （r＝0.999 9）， 0.208-52 μg/mL for toddaline （r＝0.999 9） and 0.944-236 μg/mL for sesamin （r＝0.999 9）； RSDs of precision， stability and reproducibility tests were lower than 2.0％； recoveries were 101.47％-103.92％ （RSD＝0.92％，n＝9）， 102.52％-104.68％ （RSD＝0.63％，n＝9）， 97.55％-101.22％ （RSD＝1.09％，n＝9）， 103.35％-104.93％ （RSD＝0.71％，n＝9）， 99.31％-103.86％ （RSD＝1.34％，n＝9）. CONCLUSIONS： The method is simple with good precision， stability and reproducibility， and can be used for the simultaneous determination of 5 components in Z. nitidum.