OBJECTIVE： To study the effects of Anzi mixture on Toll like receptor 4 （TLR4）/myeloid differentiation factor 88 （MyD88）/nuclear facter-κB （NF-κB） signaling pathway of antiphospholipid antibodies （APA） positive abortive mice， and to investigate the mechanism of anti-APA positive abortion. METHODS： BALB/c mice （female） were randomly divided into blank control group， model group， aspirin group （positive control， 0.019 5 g/kg） and Anzi mixture low-dose， medium-dose and high-dose groups （37.7， 75.4， 150.8 g/kg， calculated by crude drug）， with 10 mice in each group. Except for blank control group， other groups were given human β2-glycoprotein Ⅰ as derivant to establish APA positive abortion model. From the first day of pregnancy， treatment groups were given relevant medicine intragastrically， and blank control group and model group were given constant volume of normal saline intragastrically， once a day， for consecutive 9 d. mRNA and protein levels of TLR4， myeloid differentiation 2 （MD2）， MyD88 and NF-κB in  placental tissue of mice were determined by RT-PCR and immunohistochemical method. RESULTS： Compared with blank control group， mRNA and protein expression of TLR4， MD2， MyD88 and NF-κB in placental tissue were increased markedly in the model group （P＜0.01）. Compared with model group， mRNA and protein expression of TLR4， MD2 and MyD88 in aspirin group and Anzi mixture low-dose and medium-dose groups were decreased significantly as well as the protein expression of TLR4 in Anzi mixture high-dose group and the protein expression of NF-κB in all medicine groups（P＜0.05 or P＜0.01）. mRNA expression of TLR4 and MD2 and the protein expression of MD2 and MyD88 in Anzi mixture low-dose groups were lower than those in aspirin group （P＜0.05 or P＜0.01）. CONCLUSIONS： Anzi mixture can inhibit TLR4/MyD88/NF-κB signaling pathway of APA positive abortive mice， which may be one of anti-APA positive abortion mechanisms.