OBJECTIVE： To establish the HPLC fingerprint for chromatogram Yuyin tablet. METHODS： HPLC was performed on the column of Inertsil ODS-3 with mobile phase of acetonitrile-0.1％ phosphoric acid （gradient elution） at a flow rate of 1.0 mL/min， detection wavelength was 230 nm， column temperature was 30 ℃， and injection volume was 10 μL. Using quercetin as reference，the HPLC profiles of 10 batches of Yuyin tablet were detemined， Similarity Evaluation Software for Chromatographic Fingerprint of Traditional Chinese Medicine （2012 edition） was used for the common peaks iolentification and similarity evaluation. RESULTS： There were 39 common peaks in the HPLC profiles of the 10 batches of Yuyin tablet， similarity degrees were higher than 0.96. It was proved that the HPLC profiles and control fingerprint profile of 10 batches of samples had good consistency. CONCLUSIONS： The established fingerprint chromatogram  can provide reference for the identification and quality evaluation of Yuyin tablet.