OBJECTIVE： To investigate improvement effect of Duzhong zhuanggu （DZ） pills on retinoic acid-induced osteoporosis in mice. METHODS： 72 KM mice were randomized into blank control group （normal saline）， model group （normal saline）， positive control group （Xianling gubao capsule， 0.50 g/kg）， DZ pills high-dose， medium-dose and low-dose groups （1.90，0.95，0.48 g/kg， DZ-H， DZ-M， DZ-L groups）， with 12 mice in each group. Except for blank control group， other groups were given retinoic acid intragastrically to induce osteoporosis model， and given relevant medicine intragastrically once a day， for 14 days. After last administration， body weight of mice was determined； the blood sample was collected from eyeball to determine the contents of serum calcium and phosphor； liver， spleen and thymus gland were collected to determine organ index； left femur was collected to determine bone wet weight， bone dry weight， length and diameter； right femur was collected to determine the area percentage， width and number of bone trabecula. RESULTS： Compared with blank control group， the body weight of mice in model group were decreased； the contents of serum calcium and phosphorus were increased； liver indexes and  spleen indexes were increased， while thymus indexes were decreased； bone wet weight， bone dry weight， length and diameter were decreased； area percentage， width and number of trabecular were decreased， with statistical significance （P＜0.05 or P＜0.01）. Compared with model group， the contents of serum calcium were decreased in treatment groups， and the contents of serum phosphorus were decreased in positive control group， DZ-M and DZ-L groups. Thymus indexes were increased in positive control group， DZ-M and DZ-L groups， while liver indexes were decreased in positive control group； femur wet weight of positive control group as well as femur dry and wet weight， diameters of DZ-M were all increased. The percentage， width and number of bone trabecula were increased in positive control group， DZ-H and DZ-M group， and the number of bone trabecula was increased in DZ-L group， with statistical significance （P＜0.05 or P＜0.01）. CONCLUSIONS： DZ pills have improvement effect on osteoporosis induced by retinoic acid in mice.