OBJECTIVE： To provide reference for rational drug use. METHODS： The etiological data of 283 patients with brainstem hemorrhage were collected from our hospital during Jan. 2014-Dec. 2015. The distribution characteristics and drug resistance of nosocomial infection pathogens were analyzed retrospectively. RESULTS： Of 283 patients， 205 patients were diagnosed as nosocomial infection with infection rate of 72.44％. 765 clinical specimens were tested， mainly including sputum （60.00％）， blood （14.38％）， bile （9.54％）， etc. All of 765 pathogens were isolated， mainly including 496 strains of Gram-negative bacteria（64.84％） composed mainly by Klebsiella pneumoniae， 238 strains of Gram-positive bacteria（31.11％） composed mainly by Staphylococcus aureus， 31 strains of fungus （4.05％） composed mainly by Candida albicans. There were 89 strains of ESBLs-producing K. pneumoniae， 23 strains of ESBLs-producing Escherichia coli and 87 strains of MRSA. K. pneumoniae， E. coli， Pseudomonas aeruginosa and Acinetobacter baumannii were sensitive to carbapenems （resistant rate＜5％）. S. aureus， Staphylococcus epidermidis and Staphylococcus chromogenes showed high resistant rate to commonly used antibiotics， but were sensitive to vancomycin （resistant rate was 0）. CONCLUSIONS： Main nosocomial infection pathogens of ICU patients with brainstem hemorrhage in our hospital are Gram-negative bacteria， and the situation of drug resistance and enzyme production are not optimistic. Main pathogens are sensitive to carbapenems and vancomycin， etc. More attention should be paid to drug resistance characteristics of pathogen monitoring， and antibiotics should be selected rationally.