OBJECTIVE： To provide references for rational use of human serum albumin. METHODS： The inpatients receiving human serum albumin in the hospital during Jan.-Dec. 2014 were investigated retrospectively and analyzed in respects of general information of patients， serum albumin level before medication， drug dosage， drug cost and reasons. RESULTS： Eight thousand seven hundred and sixty patients from top 8 departments in the list of the number of patients of human serum albumin were included in the study， accounting for 64％ of total. There were 5 244 male （59.9％） and 3 516 female （40.1％）； patient’s age mainly ranged ＞30-60 year-old （52.6％）. The number of patients of albumin was the largest in general surgery department （2 588 cases） and cardiovascular surgery department （2 348 cases）. Referring to domestic package inserts， 8 210 patients （about 93.7％） were in accordance with indications， and hypoproteinemia was the most common medication indication （35.6％）. The drug dosage per capita of ICU was the highest （152.5 g）； total drug cost was the highest in general surgery department （13 334 396.4 yuan）； treatment course per capita of infection disease department was the longest （10.7 d）. CONCLUSION： There is irrational use of human serum albumin in our hospital and difference of drug use among departments. It is necessary to strengthen human serum albumin use monitoring and establish rational medication evaluation system so as to promote standard drug use and realize rational distribution of medical resources.