OBJECTIVE： To establish a method for the simultaneous determination of polydatin， resveratrol， emodin and physcion in Reynoutria japonica Cream. METHODS： HPLC was performed on the column of Xtimate C18 with mobile phase of acetonitrile-0.1％Phosphoric acid solution （gradient elution） at a flow rate of 1.0 mL/min， the detection wavelength was 286 nm， column temperature was 30 ℃，and the injection volume was 10 μL. RESULTS： The linear range was 10-60 μg/mL for polydatin （r＝0.999 4）， 2.5-15 μg/mL for resveratrol （r＝0.999 7）， 5-30 μg/mL for emodin （r＝0.999 8） and 2-12 μg/mL for physcion （r＝0.999 6）；limit of quantitation was 0.87，0.44，0.45，0.15 μg/mL，limit of detection was 0.46，0.15，0.17，0.07 μg/mL； RSDs of precision， stability and reproducibility tests were lower than 2.0％；recoveries were 95.00％-102.00％ （RSD＝2.25％，n＝9），95.00％-103.00％ （RSD＝2.97％，n＝9），95.00％-99.56％ （RSD＝2.05％，n＝9） and 97.50％-102.50％ （RSD＝1.59％，n＝9）， respectively. CONCLUSIONS： The method is reliable， simple， and can be used for the simultaneous determination of polydatin， resveratrol， emodin and physcion in R. japonica Cream.