OBJECTIVE： To establish the fingerprints of 11 batches of Cardiospermum halicacabum from Guangxi， and detect its spectrum-effect relationship of antioxidant activity in vitro， and provide reference for revealing the material basis for its antioxidant activity. METHODS： HPLC was conducted to establish the fingerprints of 11 batches of C. halicacabum from Guangxi， the 1，1-diphenyl-2-trinitrobenzene hydrazine （DPPH） free radical scavenging method was used to determine the antioxidant activity in vitro of C. halicacabum from different origins， and bivariate correlation analysis was adopted to detect the spectrum-effect relationship of antioxidant activity in vitro. RESULTS： The similarities of 11 batches of C. halicacabum from Guangxi were all more than 0.9， and the content change of peak 2 （protocatechuic acid）， 3， 4， 12 in 16 common peaks was closely related to the DPPH free radical scavenging activity. CONCLUSIONS： The chemical composition of C. halicacabum from different origins in Guangxi is basically consistent， while there are some differences in the quality of the producing area. The protocatechuic acid （peak 2） and the unknown compounds represented by peak 3， 4， 12 may be the pharmacodynamic material basis of its antioxidant activity.