OBJECTIVE： To establish the method for the determination of oleanolic acid and ursolic acid in different medicinal parts of Tibetan medicine Pteocephalus hookeri， and compare the differences among the different parts. METHODS： The contents of oleanolic acid and ursolic acid from different medicinal parts （whole plant， aerial part， underground part） of P. hookeri were determined by UPLC-PDA. The separation was performed on Acquity UPLC HSS T3 column（150 mm×2.1 mm，1.8 μm） with mobile phase consisted of methanol-0.1 mol/L ammonium acetate （88 ∶ 12， V/V） at the flow rate of 0.2 mL/min. The detection wavelength was set at 210 nm， and column temperature was 30 ℃. The sample size was 5 μL. RESULTS： The linear ranges of oleanolic acid and ursolic acid were 10.65-1 065 μg/mL （r＝0.999 6） and 18.8-1 880 μg/mL （r＝0.999 4）， separately. The recoveries were 96.95％ （RSD＝1.24％， n＝9） and 98.12％ （RSD＝2.13％， n＝9）， separately. RSDs of precision， stability and reproducibility tests were all less than 3％. The contents of oleanolic acid and ursolic acid from different medicinal parts in P. hookeri were in descending order of aerial part＞whole plant＞underground part； the average total content of oleanolic acid and ursolic acid from whole plants was 0.35％， the aerial part reached 0.56％ and underground part was 0.09％. CONCLUSIONS： The method is rapid， accurate and reproducible， and it is suitable for the content determination of oleanolic acid and ursolic acid in different medicinal parts of Tibetan medicine P. hookeri. The contents of oleanolic acid and ursolic acid from aerial part of P. hookeri are higher than whole plant and underground part. It is suggested to use aerial parts of medicine.