OBJECTIVE： To establish a method for the bacterial endotoxin test of Ibuprofen sodium chloride injection. METHODS： Maximum noninterference concentration of sample was determined by interference test according to bacterial endotoxins gel-clot test stated in the general principles 1143 of Chinese Pharmacopoeia 2015 edition （four）. And then methodology validation was conducted. RESULTS： Samples were diluted to 1 mg/mL， which did not interfere with bacterial endotoxin test. CONCLUSIONS： The established method can be used for the bacterial endotoxin test of Ibuprofen sodium chloride injection， and the limitation of bacterial endotoxin was 0.5 EU/mL.