OBJECTIVE: To provide reference indexs for the quality evaluation of Glycyrrhetinic acid lipo-emul. METHODS:Croy-TEM was used to detect the morphology of Glycyrrhetinic acid lipo-emul, laser nano-particle size analyzer was used to determine the particle size, polydispersity index (PDI) and the zeta potential; UPLC was used to determine the drug loading of its active ingredient glycyrrhetinic acid; placing 10 d in 30 ℃, then stability was detected. RESULTS: Prepared Glycyrrhetinic acid lipo-emul was clear outline, structural integrity,roundlike and arranged closely; the mean particle size was (245.2±4.29) nm, PDI was (0.054±0.01) and the mean zeta potential was (-6.25±0.54) mV; the average drug loading of glycyrrhetinic acid was (1.25±0.09) mg/mL; the sample with mass concentration of 0.82 mg/mL showed good stability in within 10 d. CONCLUSIONS: Particle size, zeta potential, drug loading and stability can be used as the evaluation index of Glycyrrhetinic acid lipo-emul.