OBJECTIVE： To establish a method for the simultaneous determination of 4 lignans in Schisandra chinensis， and compare the differences among different habitats. METHODS： HPLC was performed on the column of Hypersil ODS-C18 with mobile phase of acetonitrile-water （gradient elution） at a flow rate of 1.0 mL/min， the detection wavelength was 217 nm， column temperature was 30 ℃ and injection volume was 10 μL. RESULTS：The linear range was 0.375 3-2.251 8 μg for schizandrol A（r＝0.999 6），0.056 8-0.341 1  μg for schisantherin A（r＝0.999 8），0.077 4-0.464 4  μg for deoxyschizandrin（r＝0.999 3） and 0.310 5- 1.863 0  μg for schisandrin（r＝0.999 8）； RSDs of precision， stability and reproducibility were lower than 2.0％； recoveries were 97.94％-100.30％（RSD＝0.98％，n＝6），97.59％-99.61％（RSD＝0.73％，n＝6），100.86％-103.10％（RSD＝0.83％，n＝6），98.39％-101.03％（RSD＝1.03％，n＝6），respectively. CONCLUSIONS： The method is simple with good precision， stability and reproducibility， and can be used for the simultaneous determination of lignans in S. chinensis； there are quite large differences in the contents of 4 lignans in S. chinensis from different habitats.