OBJECTIVE： To analyze bacteria distribution and drug resistance of pediatric severe sepsis in our hospital， and to provide reference for clinical rational use of antimicrobial agents. METHODS： 57 pediatric severe sepsis patients were collected from pediatric intensive care unit of our hospital during Jan. 2014 to May 2015. The results of pathogen culture and drug sensitivity tests were analyzed retrospectively. RESULTS： Of 57 children， pathogen were detected in 18 cases （31.58％）. A total of 91 pathogen were detected， of which there were 24 strains of Gram-positive （G+） bacteria （26.37％） mainly including Staphylococcus and Enterococcus， 60 strains of Gram-negative （G－） bacteria （65.93％） mainly including Klebsiella pneumoniae and Acinetobacter calcoacetcus-A. baumannii complex and 7 strains of fungus （7.69％） as Candida. 4 strains of methicillin-resistant Staphylococcus， 22 strains of carbapenems-resistant K. pneumoniae， 21 strains of multi-drug resistant K. pneumoniae and 7 strains of multi-drug resistant A. calcoacetcus-A. baumannii complex were all detected. Methicillin-resistant Staphylococcus， Staphylococcus aureus and Streptococcus pneumoniae were sensitive to vancomycin and linezolid， with resistant rate of 0. K. pneumoniae was completely resistant to ampicillin sodium and sulbactam sodium， piperacillin sodium and tazobactam sodium， imipenem and cephalosporin， with resistant rate of 100％. Resistant rate of A. calcoacetcus-A. baumannii complex to major common antimicrobial agents was higher than 50％. Escherichia coli was resistant to cefotaxime， and resistant rates of other antimicrobial agents were lower than 40％. CONCLUSIONS： Main pathogen of pediatric severe sepsis is G－ bacteria in our hospital， and carbapenems-resistant K. pneumoniae is detected， to which should be pay attention. The multiple drug-resistant treatment should be adopted for pediatric severe sepsis caused by multiple drug-resistant bacteria. Antimicrobial agents should be selected rationally according to pathogen type and the results of drug sensitivity test.