OBJECTIVE： To study the pharmacokinetics and absolute bioavailability of succinyl rotundic acid （SRA） in rats. METHODS： Wistar rats were randomly divided into intragastric injection （ig） group （30 mg/kg） and tail intravenous injection （iv） group （15 mg/kg）， with 10 rats in each group. They were given SRA with relevant usage and dosage. The blood sample of tail vein 0.2 ml was respectively taken after 5，10，20，30，40，60，90，150，210，270，390，510 min of administration. The plasma concentration of SRA was determined by RP-HPLC using tanshinone ⅡA as internal standard. The pharmacokinetic parameters and absolute bioavailability were calculated by using 3p97 software. RESULTS： The pharmacokinetic parameters of ig group were that cmax was （1.02±0.27） μg/ml， tmax was （26.64±2.74） min， t1/2 was （42.79±3.46） min and AUC0-510 min was （92.27±9.26） μg·min/ml； those of tail iv group were that t1/2 was （17.21±1.58） min， and AUC0-510 min was （320.50±11.89） μg·min/ml. Oral obsolute bioavailability of SRA was 14.40％. CONCLUSIONS： The pharmacokinetic characteristics of SRA in rats conform to one-compartment model， and the absolute bioavailability is high.