OBJECTIVE： To establish the HPLC fingerprint of Pothos chinensis. METHODS： HPLC was performed on the column of Thermo Hypersil ODS-2 with mobile phase of methanol-0.4％ phosphoric acid solution （gradient elution） at a flow rate of 1.0 ml/min， detection wavelength was 300 nm， column temperature was 30 ℃，and injection volume was 10 μl. Using ferulic acid tyramine peak as reference， HPLC spectrum of 24 batches of medicinal herbs was determined， and TCM Chromatographic Fingerprint Similarity Evaluation System（2004 edition）was adopted for common peak identification and similarity evaluation. RESULTS： There were 16 common peaks totally in the 24 batches ，similarity of the 21 batches of medicinal materials were higher than 0.8. CONCLUSIONS： The established fingerprint is specific， stable， and can provide basis for quality control of P. chinensis.