OBJECTIVE： To prepare IMC-C225 modified vinorelbine （VRB） stealth cationic liposomes （called VRB cationic liposomes for short）， and to study the physicochemical properties and cell inhibition. METHODS： The VRB cationic liposomes were prepared by ammonium sulfate gradient method. The physicochemical properties， including morphology， particle size， polydispersity index （PDI）， Zeta potential， encapsulation efficiency and in vitro release rate， were studied. The inhibitory effects of the liposomes on lung caner Lewis cell were evaluated by SRB methods.  RESULTS： The prepared VRB cationic liposomes were spheroidal in shape， even in size and smooth in surface. The average particle size of liposomes was （89.06±3.56） nm， PDI value was 0.175±0.01 and Zeta potential was （35.91±0.51） mV. The encapsulation efficiency was （90.61±0.80）％， and in vitro release rate was （26.74±3.76）％  within 48 h （n＝3）. Compared with vinorelbine liposomes and IMC-C225 modified blank stealth cationic liposomes， VRB cationic liposomes could reduce the survival rate of Lewis cells. CONCLUSIONS： Prepared VRB cationic liposomes have even particle size and high encapsulation efficiency， and show significant inhibitory effect on Lewis cells.