OBJECTIVE： To investigate therapeutic efficacy and recurrence rate of vaginal administration of lactobacillus combined with chlorquinaldol-promestriene for senile patients with atrophic vaginitis. METHODS： 150 senile patients with atrophic vaginitis were selected and divided into group A， B， C according to random number table， with 50 cases in each group. Group A was given Lactobacillus vaginal capsules， 2 capsules， qd （at bedtime）； group B given Chlorquinaldol-promestriene vaginal tablets， 1 tablet， qd （at bedtime）； group C given Chlorquinaldol-promestriene vaginal tablets， 1 tablet， qd （at bedtime）， and Lactobacillus vaginal capsules， 2 capsules， qd， next morning， implanting into vagina. 3 groups received treatment for 18 d. Clinical efficacy， vaginal health score， vaginal symptom score and vaginal pH value were observed in 3 groups， and the occurrence of ADR and recurrence rate during follow-up period （12 months） were recorded. RESULTS： The total effective rate of group C was 94.00％， which was significantly higher than group A （72.00％） and B （74.00％）， with statistical significance （P＜0.05）. Before treatment， there was no statistical significance in vaginal health score， vaginal symptom score and vaginal pH value among 3 groups （P＞0.05）. After treatment， vaginal health score of 3 groups were increased significantly， while vaginal symptom score and vaginal pH value were decreased significantly； and the improvement of above indexes in group C were significantly better than in group A and B， with statistical significance （P＜0.05）. No obvious ADR was found in 3 groups during treatment. The recurrence rate of group C during follow-up period （4.00％） was significantly lower than that of group A （18.00％） and group B （20.00％）， with statistical significance （P＜0.05）. CONCLUSIONS： Vaginal administration of lactobacillus combined with chlorquinaldol-promestriene for senile patients with atrophic vaginitis can effectively relieve clinical symptoms and signs， improve vaginal cleanliness， regulate the local pH value and  reduce the risk of recurrence， with good safety.