OBJECTIVE： To establish a method for the contents determination of psoralen， isopsoralen， psoralenoside and isopsoralenoside in Danzhi qing’e tablet. METHODS： HPLC performed on the column of Eclipse XDB-C18 with mobile phase of methanol-water （51 ∶ 49，V/V） （isocratic elution， for psoralen and isopsoralen） and acetonitrile-0.1％ formic acid （12 ∶ 88，V/V） （isocratic elution， for psoralenoside and isopsoralenoside） at a flow rate of 1.0 ml/min， the detection wavelength was 246 nm， column temperature was 30 ℃， and injection volume was 10 μl. RESULTS： The linear range was 3.138-200.8 μg/ml for psoralen （r＝0.999 9）， 3.175-203.2 μg/ml for isopsoralen（r＝0.999 9），3.181-101.8 μg/ml for psoralenoside （r＝0.999 9） and 3.169-101.4 μg/ml for isopsoralenoside （r＝0.999 9）； RSDs of precision， stability and reproducibility tests were lower than 2％； limits of quantitation were 0.627 5 ng， 0.635 0 ng， 3.181 0 ng and 3.169 0 ng，the limits of detection were 0.251 0 ng， 0.254 0 ng， 1.273 0 ng and 1.268 0 ng； recoveries were 95.68％-102.80％（RSD＝2.4％，n＝6）， 95.91％-102.10％（RSD＝2.3％，n＝6）， 98.64％-99.13％（RSD＝0.23％，n＝6） and 100.20％-101.70％（RSD＝0.69％，n＝6）， respectively. CONCLUSIONS： The method is simple and accurate， and can be used for the simultaneous determination of psoralen， isopsoralen， psoralenoside and isopsoralenoside in Danzhi qing’e tablet.