OBJECTIVE： To establish a method for the content determination of erythromycin ethylsuccinate in Erythromycin ethylsuccinate tablet. METHODS：The column of Dikma-C18 with mobile phase of acetonitrile-0.05％ diethylamine（70 ∶ 30，V/V） at a flow rate of 1.0 ml/min， column temperature was 30 ℃， and the sample size was 10 μl. RESULTS：The linear range of erythromycin ethylsuccinate was 1.216-7.296 μg （r＝0.999 2）； limit of quantitation was 0.304 μg， the limits of detection was 0.076 μg， respectively；the RSDs of precision， stability and reproducibility tests were no more than 2.0％， the average recoveries was 97.59％-104.19％（RSD＝1.71％，n＝6）. CONCLUSIONS： The method is simple and accurate with high sensitivity and reproducibility， and can be used for the content determination of Erythromycin ethylsuccinate tablet.