OBJECTIVE： To establish a method for the contents determination of Li， Be， Mg， Al， V， Cr， Mn， Fe， Co， Ni， Cu， Zn， As， Se， Rb， Sr， Ag， Cd， Cs， Ba， Hg， Pb and U in Jiangu injection. METHODS： Microwave digestion-inductively coupled plasma mass spectrometry （MD-ICPMS） was adopted. RF power was 1 550 W， sampling depth was 8 mm， coolant temperature was 2 ℃， collision gas was He gas， the flow rate of the carrier gas （argon gas） was 1.03 L/min， integration time was 0.5 s， plasma gas flow rate was 15 L/min ，molecular turbo pumb speed was 200 L/s，quadrupole degree of 1.75 ×10－3 Pa，sampling cone aperture was 1.0 mm，interception cone aperture was 0.4 mm，and the data collection was repeated 3 times. RESULTS： The linear ranges of Li， Be， Mg， Al， V， Cr， Mn， Fe， Co， Ni， Cu， Zn， As， Se， Rb， Sr， Ag， Cd， Cs， Ba， Pb and U were all in 0-100 ng/ml （r≥0.999 6）， Hg was 0-2.0 ng/ml （r≥0.999 6）； the limit of detection was 0.005-1.652 μg/L； RSDs of precisionand reproducibility tests were lower than 7％；the accuracy determined by the instrument in the test was good； recoveries were 90.03％-96.11％，88.25％-96.76％，83.54％-92.01％， 85.02％-99.18％，91.87％-94.62％，90.31％-92.28％， 89.82％-96.99％，83.45％-89.56％，88.55％-92.39％， 92.68％-95.18％，89.04％-93.81％，94.76％-103.11％，96.76％-101.39％，98.36％-105.27％，106.97％-119.89％， 97.36％-103.46％，89.11％-90.73％，92.37％-95.62％， 95.78％-98.22％，91.59％-97.18％，93.93％-97.12％，96.86％-99.22％ and 106.14％-110.07％，RSD＝0.48％～4.24％，n＝6. CONCLUSIONS： The method is simple， accurate， and suitable for the simultaneous determination of 23 trace elements in Jiangu injection.