OBJECTIVE： To establish a method for simultaneous determination of magnolol and honokiol in Guipo pellet. METHODS： HPLC was performed on the column of Alltima C18 with mobile phase of methanol-water （70 ∶ 30，V/V） at a flow rate at 1.0 ml/min， detection wavelength was 294 nm， column temperature was 35 ℃ and injection volume was 10 μl. RESULTS： The linear range was 0.206 4-2.064 μg for magnolol （r＝0.999 8） and 0.088 0-0.880 μg for honokiol （r＝0.999 9）； limits of quantitation were 0.41， 0.26 μg/ml， detection limits were 0.12， 0.075 μg/ml， respectively； RSDs of precision， stability and reproducibility tests were lower than 2.0％； recoveries were 99.86％-102.11％ （RSD＝1.02％，n＝6） and 96.53％-101.74％ （RSD＝1.83％，n＝6）. CONCLUSIONS： The method is accurate， sensitive， reproducible， and suitable for the simultaneous determination of magnolol and honokiol in Guipo pellet.