OBJECTIVE： To establish a method for the simultaneous determination of acetic acid and succinic acid in Banxia syrup. METHODS： RP-HPLC was performed on the column of GL InterSustain-C18 with mobile phase of 0.03 mol/L ammonium dihydrogen phosphate buffer solution （pH2.0）-methanol （gradient elution） at a flow rate of 0.8 ml/min， the detection wavelength was 210 nm， column temperature was 30 ℃， injection volume was 10 μl. RESULTS： The linear range was 0.020 98-0.209 8 μg/ml for acetic acid （0.999 9）and 12.04-120.4 μg/ml for succinic acid（r＝0.999 9）； limits of quantification were 0.15， 18.24 ng， limits of detection were 0.045， 5.53 ng； RSDs of precision， stability and reproducibility tests were lower than 2％； recoveries were 97.45％-101.68％ （RSD＝1.39％，n＝9） and 98.31％-101.08％ （RSD＝1.01％，n＝9）. CONCLUSIONS： The method is accurate and rapid， and suitable for the simultaneous determination of acetic acid and succinic acid in Banxia syrup.