OBJECTIVE： To evaluate the situation and rationality of the implementation of antimicrobial therapy for patients with acute pancreatitis （AP）in our hospital. METHODS： 132 AP patients admitted into our hospital during Aug. 2013-Dec. 2015 were selected and analyzed statistically in respects of the types of antimicrobial agents， drug combination， usage and dosage， etc. The rationality of antimicrobial agents use was also analyzed. RESULTS： Among 132 AP patients， there were 103 men and 29 women； mean age of them was （43.75±1.26） years； mild acute pancreatitis （MAP， 117 cases） accounted for 88.64％， and severe acute pancreatitis （SAP， 15 cases） accounted for 11.36％； the top 3 causes of AP were obstruction factors （32.31％）， hyperlipidermia （27.21％） and alcohol （18.03％）， respectively. Main symptoms were abdominal pain， abdominal distension and vomiting； among 132 AP patients， 101 cases were cured and 31 cases were recovered. The antimicrobial agents were used in 107 MAP cases and 13 SAP cases， and the total utilization ratio was 90.91％ （of which， 90.15％ belonged to therapeutic drug use）. A total of 5 species were used， the combination proportion of which was 77.28％， among which two-drug combination was the most （69.70％）， mainly including “sulbactam sodium and cefoperazone+tinidazole” （45.65％） and “levofloxacin+tinidazole” （27.17％）. After treatment， patient’s serum amylase level MEUT and WBC recovered to normal range. CONCLUSIONS： The utilization of antimicrobial agents in our hospital is basic reasonable for AP patients， meat the requirements of the releveant guidelines. The rationality of prophylactic use of agents remain to be discussed.