OBJECTIVE： To establish a method for the rapid determination of ligustroflavone in Fructus ligustri. METHODS： HPLC was conducted to determine the content of ligustroflavone （as reference value）： the column was Diamonsil C18 with mobile phase of acetonitrile-water （gradient elution） at a flow rate of 1 ml/min， the detection wavelength was 230 nm， column temperature was 30℃ and the injection volume was 10 μl. Partial least square （PLS）-near infrared spectroscopy （NIRS） were adopted to establish the quantitative calibration model of ligustroflavone content： according to the determination results of ligustroflavone content， 72 samples of calibration set and 24 samples of validation set were collected and pretreated spectrum by multivariate scattering correction （MSC） combined with the First Derivative， the optimized wavelength band was 9 999.89-4 001.01 cm－1 and principal component factor number was 9. RESULTS： Methodology validation of content determination for ligustroflavone was in line with the requirement. Internal cross-validation coefficient of the quantitative calibration model for ligustroflavone was 0.984 69， corrected root mean square deviation was 0.097， the root mean square error of predication was 0.100， and the root mean square error of cross validation was 0.240 13. There was no significant different between the predicted value and the measured value. CONCLUSIONS： The method is rapid， accurate， simple without destruction and pollution， and can be used for the rapid determination of ligustroflavone in F. ligustri.