OBJECTIVE： To establish the bacterial endotoxin test method of Ossotide injection. METHODS： According to the appendix of the gel method under “bacterial endotoxin test method” in Chinese Pharmacopiea （2015 edition）， the maximal non-interference concentrations of samples were determined by interference test， and then methodology validation was conducted. RESULTS： The agglutination of LAL with bacterial endotoxin had no interference when the sample was diluted to 0.333 mg/ml or below. CONCLUSIONS： The established method can be used to detect the bacterial endotoxin of Ossotide injection.