OBJECTIVE： To study the  chemical compositions of n-butabol extract from Solanum lyratum. METHODS： Glucan LH-20 column chromatography， silica gel column chromatography and TLC were adopted to separate and purity the chemical compositions， physicochemical property and spectral evidence to identify their  structures. RESULTS： Totally 10 chemical compositions were separated from n-butabol extract， namely apigenin-7-O-β-D-apiofuanosyl（1→2）-β-D-glucose （1），apigenin-7-O-β-D-glucose （2），adenosine （3），3-methoxy-4-hydroxy-5-[（8′ S）-3′ -methoxy-4′ -hydroxyl-phenyl-alcohol]-E-cinnamic-phenylpropyl alcohol-4-O- β-D-glucoside （4），N-（4-amino-butyl）-3-（3-hydroxy-4-methoxy-phenyl）-E-acrylamide （5），N-（4-amino-butyl）-3-（3-hydroxy-4-methoxy-phenyl）-Z-acrylamide （6）， resveratrol （7）， naringenin （8）， quercetin （9） and dioscin （10）. CONCLUSIONS： Compound 1-8 are first separated from S. lyratum， the study can lay a foundation for quality evaluation of S. Lyratum.