OBJECTIVE： To establish a method for the simultaneous determination of 5 anthraquinones （aloe emodin， rhein， emodin， chrysophanol and physcion） in Baoshenyi pill. METHODS： HPLC was conducted， using emodin as reference peak， the relative correction factors （RCFS） between emodin and aloe emodin， rhein， chrysophanol and physcion were calculated to calculate the contents of above-mentioned 5 anthraquinones. The column was Waters Symmetry C18 with mobile phase of methanol-0.1％ phosphoric acid solution （70 ∶ 30，V/V） at a flow rate of 1.0 ml/min， the detection wavelength was 254 nm， column temperature was 30 ℃ and injection volume was 10 μl. RESULTS： The linear range was 5.83-186.6 μg/ml for aloe emodine（r＝0.999 7）， 9.39-300.5 μg/ml for rhein（r＝0.999 5），9.55-305.6 μg/ml for emodin（r＝0.999 9），10.23-327.4 μg/ml for chrysophanol（r＝0.999 9） and 4.01-128.4 μg/ml physcion （r＝0.999 4）； the limits of quantification were 364.4，880.2，596.9，319.7，501.3 ng， limits of detection were 109.3，293.4，179.1，95.9，150.4 ng； RSDs of precision， stability and reproducibility tests were lower than 2.0％； recoveries were 96.38％-100.90％（RSD＝1.62％，n＝6）， 97.72％-101.75％（RSD＝1.44％，n＝6）， 97.66％-102.34％ （RSD＝1.63％，n＝6）， 97.33％-101.91％（RSD＝1.75％，n＝6） and 97.78％-100.74％（RSD＝1.19％，n＝6）. The RCFs of emodin to aloe emodine， rhein， chrysophanol and physcion were 1.217 7， 1.153 4， 1.424 2 and 1.034 5， respectively. CONCLUSIONS： The method is reliable， simple and fast， and can be used for the simultaneous determination of aloe emodin， rhein， emodin， chrysophanol and physcion in Baoshenyi pill.