OBJECTIVE： To establish a method for simultaneous determination of ephedrine hydrochloride， pseudoephedrine hydrochloride， brucine， strychnine in Rheumatoid arthritis tablet. METHODS： HPLC was performed on column of XTerra-C18 with mobile phase of acetonitrile-mixed solution （pH was adjusted to 2.8 with phosphate acid） composing of 0.01 mol/L sodium heptanesulfonate solution and equal quantity of 0.01 mol/L potassium dihydrogen phosphate solution at a flow rate of 1.0 ml/min， detection wavelength was 206 nm for ephedrine hydrochloride and pseudoephedrine hydrochloride， 260 nm for brucine and strychnine， column temperature was 30℃， and injection volume was 10 μl. RESULTS： The linear range was 0.916-18.320 0 μg/ml for pseudoephedrine hydrochloride （r＝0.999 6），10.034-200.680 0 μg/ml for ephedrine hydrochloride（r＝0.999 7），8.693-121.700 0 μg/ml for brucine（r＝0.999 9） and 9.696-193.920 0 μg/ml for strychnine（r＝0.999 9）； limits of quantification were 0.03， 0.07， 0.04， 0.03 ng，limits of detection were  0.01， 0.02，0.01， 0.01 ng； RSDs of precision， stability and reproducibility tests were lower than 2.0％； recoveries were  97.30％-99.79％（RSD＝0.96％，n＝6），95.81％-99.36％（RSD＝0.38％，n＝6）， 95.34％-99.93％ （RSD＝2.00％，n＝6） and 95.59％-99.80％（RSD＝1.76％，n＝6）. CONCLUSIONS： The method is simple， accurate， and can be used for the simultaneous determination of ephedrine hydrochloride， pseudoephedrine hydrochloride， brucine， strychnine in Rheumatoid arthritis tablet.