OBJECTIVE： To optimize and improve the quality standard for Compound pediatric anticough oral solution. METHODS： TLC was adopted for the qualitative identification of Glycyrrhiza uralensis； chemical reaction was adopted for the qualitative identification of ipecac tincture， ammonium chloride； and HPLC was adopted for the content determination of ammonium glycyrrhizinate： the column was Agilent Zorbax XDB C18 with mobile phase of acetonitrile-0.2 mol/L ammonium acetate （20 ∶ 80，V/V） at a flow rate of 1.0 ml/min， the detection wavelength was 250 nm， column temperature was 30 ℃， and the injection volume was 10 μl. RESULTS： TLC spots of G. uralensis were clear and well-separated， negative control without interference. Differential response of ipecac tincture and ammonium chloride showed significant positive characteristics. The linear range of ammonium glycyrrhizinate was 6.08-72.96 μg/ml （r＝0.999 9）； RSDs of precision， stability and reproducibility tests were lower than 1.0％； recovery was 99.69％-100.28％ （RSD＝0.20％，n＝6）. CONCLUSIONS： Optimizing and improving the standard are helpful for the quality control of Compound pediatric anticough oral solution.