OBJECTIVE: To establish the quality standard of ultramicro decoction piece of Meng medicine Gardenia jasminoides. METHODS: Microscopic identification character of the ultramicro decoction piece was observed; TLC was adopted for its qualitative identification; moisture, ash, extract were detected; HPLC was used for the content determination of geniposide: the column was Kromasil C18 with mobile phase of acetonitrile-water (15 ∶ 85,V/V) at a flow rate of 1.0 ml/min, detection wavelength was 238 nm, column temperature was 30 ℃, and injection volume was 10 μl. RESULTS: Cell wall was completely broken and the particle sizes were 5-60 μm. TLC spots were clear and well separated. The moisture was 3.89%-5.55%, ash was 3.13%-5.22%, acid-insoluble ash was 0.16%-0.80%, and extract was 29.2%-43.4%. The linear range of geniposide was 1.56-7.82 μg/ml (r=0.999 9); RSDs of precision, stability and reproducibility tests were lower than 2.0%; recovery was 100.17%-102.04% (RSD=1.07%,n=6). CONCLUSIONS: The standard can be used for the quality control of ultramicro decoction piece of Meng medicine G. jasminoides.
关键词
蒙药大栀子超微饮片栀子苷质量标准
Keywords
Gardenia jasminoidesUltramicro decoction pieceGeniposideQuality standard