OBJECTIVE： To establish a method for the contents determination of lidocaine hydrochloride， chlorpheniramine maleate and chloramphenicol in Kemin cream. METHODS： HPLC was performed on the column of WondaSil C18 with mobile phase of methanol-acetonitrile-0.1％ phosphoric acid （gradient elution） at a flow rate of 1.0 ml/min， detection wavelength was 254 nm， column temperature was 25 ℃ and injection volume was 10 μl. RESULTS： The linear range was 0.604 5-2.871 4 μg for lidocaine hydrochloride（r＝0.999 9），0.169 9-0.807 0 μg for chlorpheniramine maleate（r＝0.999 9） and 0.326 5-1.550 9 μg for chloramphenicol （r＝0.999 9）； limit of quantitation was 3.17，1.50，2.33 ng， limit of detection was 0.95，0.45，0.70 ng； RSDs of precision， stability and reproducibility tests were lower than 2％； recoveries were 98.29％-100.10％（RSD＝0.64％，n＝9）， 98.04％-100.70％（RSD＝0.80％，n＝9） and 98.02％-99.81％（RSD＝0.70％，n＝9）， respectively. CONCLUSIONS： The method is accurate with high precision， and can be used for the simultaneous determination of lidocaine hydrochloride， chlorpheniramine maleate and chloramphenicol in Kemin cream.